The outbreaks of pseudorabies have been frequently reported in Bartha-K61-vaccinated farms in China since 2011. To study the\npathogenicity and evolution of the circulating pseudorabies viruses in Fujian Province, mainland China, we isolated and sequenced the\nwhole genome of a wild-type pseudorabies virus strain named ââ?¬Å?FJ-2012.ââ?¬Â We then conducted a few downstream bioinformatics\nanalyses including phylogenetic analysis and pathogenic analysis and used the virus to infect 6 pseudorabies virus-free piglets. FJ-2012-\ninfected piglets developed symptoms like high body temperature and central nervous system disorders and had high mortality rate. In\naddition, we identified typical micropathological changes such as multiple gross lesions in infected piglets through pathological\nanalysis and conclude that the FJ-2012 genome is significantly different from known pseudorabies viruses, in which insertions,\ndeletions, and substitutions are observed in multiple immune and virulence genes. In summary, this study shed lights on the molecular\nbasis of the prevalence and pathology of the pseudorabies virus strain FJ-2012.The genome of FJ-2012 could be used as a reference to\nstudy the evolution of pseudorabies viruses, which is critical to the vaccine development of new emerging pseudorabies viruses.
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